Anti-IL1 beta antibody

• Supplier: Abcam
• Catalog: ab2105
• Prices: $403.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ELISA IHC-P RIA IHC-F FA FC B/N ICC/IF ICC/IF
• Species Reactivities: Human, Primate
• Antigen: Recombinant 153 aa human IL-1beta produced in E
• Description: Rabbit Polyclonal
• Gene: IL1B
• Conjugate: Unconjugated

Product images

ab2105 staining IL1 beta in Human monocytes by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% saponin in PBS. The sample was incubated with the primary antibody (1/500 in 0.1% saponin + 1% FCS in PBS) for 45 minutes at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.1 - unstimulated monocytes. 2 - monocytes stimulated with LPS.See Abreview

All lanes : Anti-IL1 beta antibody (ab2105) at 1/1000 dilutionLane 1 : Human peripheral blood mononuclear cell lysate - PBMC's unstimulatedLane 2 : Human peripheral blood mononuclear cell lysate - monocytes unstimulatedLane 3 : Human peripheral blood mononuclear cell lysate - monocytes stimulated with LPSLane 4 : Human peripheral blood mononuclear cell lysate - positive control (rh IL-1 beta protein)Lysates/proteins at 25 µg per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.Observed band size : 35 kDa (why is the actual band size different from the predicted?)Exposure time : 10 minutesThis image is courtesy of an anonymous AbreviewSee Abreview

Lane 1 : Lanes 2 - 5 : Anti-IL1 beta antibody (ab2105) at 1/1000 dilutionLane 1 : Ladder (top to bottom: p35, p25, p20, p17, p11)Lane 2 : Human THP-1 leukemic monocyte cell lysate, no LPS at 20 µgLane 3 : Human THP-1 leukemic monocyte supernatant, no LPS at 20 µgLane 4 : Human THP-1 leukemic monocyte cell lysate, LPS stimulation at 20 µgLane 5 : Human THP-1 leukemic monocyte supernatant, LPS stimulation at 20 µgSecondaryLanes 2 - 5 : IRDye® 800CW-conjugated Donkey anti-rabbit IgG monoclonal at 1/5000 dilutionPerformed under reducing conditions.Observed band size : 17,30 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewBoth mature and pro-IL-1beta bands, p17 and p31 were detected by ab2105 in supernatant from THP-1 cells stimulated with LPS. Only pro-IL-1beta band (p31) was detected in supernatant from THP-1 cells not stimulated with LPS.See Abreview

ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.See Abreview

ab2105 staining IL1 beta in human keratinocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol, permeabilized in 0,1% Saponin / PBS and then blocked using 4% BSA for 30 minutes at 25°C. Samples were then incubated with ab2105 at a 1/100 dilution for 2 hours at 4°C. An Alexa-fluor 488 conjugated goat anti-rabbit polyclonal was used as the secondary antibody at a 1/100 dilution.See Abreview

ab2105 staining IL1 beta in rat alveolar macrophage tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with blocking agent for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours. An HRP-conjugated rabbit monoclonal IgG (1/500) was used as the secondary antibody. See Abreview

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