Anti-IL10 antibody
| Name | Anti-IL10 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab95062 |
| Prices | $376.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide selected from the center region of Human IL10 (NP_000563) conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | IL10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab95062 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95062 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-IL10 antibody (ab95062) at 1/100 dilution (in TTBS/1% NFDM for 1 hr. at room temperature) + MDA-MB435 cell lysate at 35 µgSecondaryEnzyme-linked secondary antibody (typically horseradish peroxidase or alkaline phosphatase) in TTBS/1% NFDM for 30 min. at room temperature.