All lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilutionLane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µgLane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µgLane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µgLane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µgLane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µgLane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µgLane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µgSecondaryHRP conjugated goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.Observed band size : 10 kDa (why is the actual band size different from the predicted?)Additional bands at : 26 kDa (possible dimer).Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Miss Fenny ChongSee
IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.