Anti-IMPDH2 antibody [EPR8365(B)]

• Supplier: Abcam
• Catalog: ab129165
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8365(B)
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on the C terminal of Human IMPDH2
• Description: Rabbit Monoclonal
• Gene: IMPDH2
• Conjugate: Unconjugated

Product images

Overlay histogram showing K562 cells stained with ab129165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129165, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : A549 cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

ab129165, at 1/250 dilution, staining IMPDH2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

ab129165, at 1/250 dilution, staining IMPDH2 in MCF7 cells by Immunofluorescence.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD