Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]

• Supplier: Abcam
• Catalog: ab76157
• Prices: $381.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1230Y
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues within human Indoleamine 2, 3-dioxygenase
• Description: Rabbit Monoclonal
• Gene: IDO1
• Conjugate: Unconjugated

Product images

ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/5000 dilution + HeLa cell lysate treated with IFN gamma at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution

Product References

Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in - Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in

Yamazaki T, Matsuo J, Nakamura S, Oguri S, Yamaguchi H. J Infect Chemother. 2014 Aug;20(8):460-4.

Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide - Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide

De Ravin SS, Zarember KA, Long-Priel D, Chan KC, Fox SD, Gallin JI, Kuhns DB, Malech HL. Blood. 2010 Sep 9;116(10):1755-60.