ab56057 staining Inhibin in human testicular cancer (seminoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked for 20 minutes at 26°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Anti-Inhibin beta A antibody (ab56057) at 1/1000 dilution + Human COV434 whole cell lysate at 60 µgSecondaryIRDye® 680LT-conjugated goat anti-rabbit IgG polyclonal at 1/25000 dilution
ab56057 staining Inhibin beta A in human COV434 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with ethanol and blocked for 3 hours at 26°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 21°C. A IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/15000) was used as the secondary antibody.See Abreview