Anti-Integrin alpha 2+beta 1 antibody [16B4]

• Supplier: Abcam
• Catalog: ab30483
• Prices: $391.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 16B4
• Applications: ICC/IF ICC/IF IP FC ELISA IHC-F
• Species Reactivities: Human
• Antigen: Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract
• Description: Mouse Monoclonal
• Gene: ITGA2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ICC/IF image of ab30483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.

ab30483 staining human breast cancer cells by ICC/IF.  Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30µg/ml) for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.See Abreview

Product References

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Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA - Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA

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Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and - Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and

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PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the - PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the

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