Immunoflouorescent analysis of HepG2 cells labelling Isocitrate dehydrogenase with ab135659 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling Isocitrate dehydrogenase with ab135659 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
All lanes : Anti-Isocitrate dehydrogenase antibody (ab135659) at 1/50 dilutionLane 1 : HepG2 cell lysateLane 2 : Mouse liver tissue lysateLysates/proteins at 35 µg per lane.
Flow cytometric analysis of 293 cells labelling Isocitrate-dehydrogenase with ab135659 at 1/10 dilution (green) or negative control cell (blue), using FITC-conjugated goat anti-rabbit secondary antibodies.