![Overlay histogram showing HEK293 cells stained with ab117976 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117976, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10385_ab117976-7-ab117976FC.jpg)
Overlay histogram showing HEK293 cells stained with ab117976 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117976, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Isocitrate dehydrogenase antibody [2H9] (ab117976) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY Isocitrate dehydrogenase Lysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10386_Isocitrate-dehydrogenase-Primary-antibodies-ab117976-1.jpg)
All lanes : Anti-Isocitrate dehydrogenase antibody [2H9] (ab117976) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY Isocitrate dehydrogenase Lysates/proteins at 5 µg per lane.
![All lanes : Anti-Isocitrate dehydrogenase antibody [2H9] (ab117976)Lane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10387_Isocitrate-dehydrogenase-Primary-antibodies-ab117976-2.jpg)
All lanes : Anti-Isocitrate dehydrogenase antibody [2H9] (ab117976)Lane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
Immunohistochemical staining of paraffin embedded Human breast tissue, using ab117976 at a dilution of 1/50.
Immunohistochemical staining of paraffin embedded Human colon tissue, using ab117976 at a dilution of 1/50.
Immunohistochemical staining of paraffin embedded Human endometrium tissue, using ab117976 at a dilution of 1/50.
Immunofluorescent staining of COS7 cells transiently transfected by Isocitrate dehydrogenase, using ab117976 at a dilution of 1/100.