All lanes : Anti-Isocitrate dehydrogenase antibody - N-terminal (ab135658) at 1/50 dilutionLane 1 : Mouse liver tissue lysateLane 2 : 293 cell line lysateLysates/proteins at 35 µg per lane.
Immunofluorescent analysis of HepG2 cells labelling Isocitrate dehydrogenase with ab135658 at a 1/10 dilution, followed by an Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of 293 cells labelling Isocitrate dehydrogenase with ab135658 at a 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling Isocitrate dehydrogenase with ab135658 at 1/50 dilution .followed by peroxidase-conjugated secondary antibody and DAB staining.