All lanes : Anti-KAT3B / p300 antibody - ChIP Grade (ab10485) at 1/5000 dilutionLane 1 : Human MCF-7 Cell LysateLane 2 : MDA-MB-231 Cell LysateLysates/proteins at 50000 cells per lane.SecondaryAnti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866)developed using the ECL techniquePerformed under reducing conditions.Exposure time : 3 minutesThis image is courtesy of an anonymous AbreviewBlocking Solution and Diluent 5% milk in TBSSee Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KAT3B / p300 with ab10485 at 1/1000 (1µg/ml). Detection: DAB.
ab10485 detecting p300 in HeLa cells by Immunoprecipitation .Left-hand panel represents a coomassie-blue stained gel loaded with 10mg of HeLa cytosolic (S100) and nuclear (NE) extracts;For the Immunoprecipitation experiment (right-hand panel), ab10485 was used at a concentration of 20 µg/10 mg of HeLa whole cell lysate (lanes correspond to 5, 15 and 50 µg of lysate). The flow-through was analysed by WB using ab10485 at a concentration of 0.04 µg/ml. The identity of the band marked as "p300" was also confirmed using mass spectrometry.