Anti-KMT2A / MLL antibody [mmN4.4]
| Name | Anti-KMT2A / MLL antibody [mmN4.4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32400 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mmN4.4 |
| Applications | IHC-P FC IP WB ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant MBP fusion protein containing residues 161 - 356 of Human MLL |
| Description | Mouse Monoclonal |
| Gene | KMT2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-KMT2A / MLL antibody [mmN4.4] (ab32400) at 10 µg/ml + Lysate from transfected cells overexpressing KMT2A/MLL SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13754_ab32400_1.jpg)
Anti-KMT2A / MLL antibody [mmN4.4] (ab32400) at 10 µg/ml + Lysate from transfected cells overexpressing KMT2A/MLL SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
![Overlay histogram showing HeLa cells stained with ab32400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32400, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13755_KMT2A-MLL-Primary-antibodies-ab32400-1.jpg)
Overlay histogram showing HeLa cells stained with ab32400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32400, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC image of ab32400 staining in human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Expression of p300 and CBP is associated with poor prognosis in small cell lung - Expression of p300 and CBP is associated with poor prognosis in small cell lung
Gao Y, Geng J, Hong X, Qi J, Teng Y, Yang Y, Qu D, Chen G. Int J Clin Exp Pathol. 2014 Jan 15;7(2):760-7. eCollection 2014.
miR-9 is an essential oncogenic microRNA specifically overexpressed in mixed - miR-9 is an essential oncogenic microRNA specifically overexpressed in mixed
Chen P, Price C, Li Z, Li Y, Cao D, Wiley A, He C, Gurbuxani S, Kunjamma RB, Huang H, Jiang X, Arnovitz S, Xu M, Hong GM, Elkahloun AG, Neilly MB, Wunderlich M, Larson RA, Le Beau MM, Mulloy JC, Liu PP, Rowley JD, Chen J. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11511-6. doi:
Menin critically links MLL proteins with LEDGF on cancer-associated target genes. - Menin critically links MLL proteins with LEDGF on cancer-associated target genes.
Yokoyama A, Cleary ML. Cancer Cell. 2008 Jul 8;14(1):36-46.
The menin tumor suppressor protein is an essential oncogenic cofactor for - The menin tumor suppressor protein is an essential oncogenic cofactor for
Yokoyama A, Somervaille TC, Smith KS, Rozenblatt-Rosen O, Meyerson M, Cleary ML. Cell. 2005 Oct 21;123(2):207-18.
Leukemia proto-oncoprotein MLL forms a SET1-like histone methyltransferase - Leukemia proto-oncoprotein MLL forms a SET1-like histone methyltransferase
Yokoyama A, Wang Z, Wysocka J, Sanyal M, Aufiero DJ, Kitabayashi I, Herr W, Cleary ML. Mol Cell Biol. 2004 Jul;24(13):5639-49.