Anti-KRIT1 antibody [EPR16560]

• Supplier: Abcam
• Catalog: ab196025
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16560
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment within Human KRIT1 aa 1-150
• Description: Rabbit Monoclonal
• Gene: KRIT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysateLane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution + HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell line) cells labeling KRIT1 with ab196025 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HUVEC cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab196025 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.