Anti-Laminin antibody

• Supplier: Abcam
• Catalog: ab30320
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-F IHC-F
• Species Reactivities: Mouse, Rat, Mammalian
• Antigen: Full length native protein isolated from mouse EHS tumor(purified)
• Description: Rabbit Polyclonal
• Gene: Lama1
• Conjugate: Unconjugated

Product images

Rat peripheral nerve (low and high magnification; formalin fixed, frozen sections). Immuno-FITC stain (color inverted) with 1:1000 anti-Laminin1 antibody (ab30320).

ICC/IF image of ab30320 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Tumour PDGF-BB expression levels determine dual effects of anti-PDGF drugs on - Tumour PDGF-BB expression levels determine dual effects of anti-PDGF drugs on

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Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and - Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and

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