ab37619 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37619 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-MADM antibody - Aminoterminal end (ab37619) at 1/100 dilutionLane 1 : HL-60 cell lysateLane 2 : mouse brain tissue lysateSecondaryHRP-anti-rabbit visualised with chemiluminescence.
ab37619 at 1/50 staining Human cancer tissue (hepatocarcinoma) by IHC-P