Anti-MAP2 antibody
| Name | Anti-MAP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5392 |
| Prices | $401.00 |
| Sizes | 25 µl |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Applications | ELISA IHC-F IHC-F IHC-P WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Bovine, Human, Monkey, Marmoset |
| Antigen | Cow brain MAP 2 purified from the material that pellets with GTP polymerized tubulin (see references) |
| Description | Chicken Polyclonal |
| Gene | MAP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain. An AlexaFluor® 633 conjugated goat anti-chicken antibody was used as the secondary.See Abreview
ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.See Abreview
ab5392 staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).Boiling in citrate-buffer was used as antigen retrieval method. ab5392 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.See Abreview
ab5392 detecting MAP2 recombinant protein by direct ELISA. Mouse recombinat protien was coated on to microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C, Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An alkaline phosphatase Goat anti-chicken IgG polyclonal (1/100000) was used as the secondary antibody.See Abreview
Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µgSecondaryHRP-conjugated rabbit anti-chicken polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 70,280 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview
Product References
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