Anti-Mast Cell Chymase antibody [CC1]
| Name | Anti-Mast Cell Chymase antibody [CC1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2377 |
| Prices | $391.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | CC1 |
| Applications | IHC-P IHC-F WB FC |
| Species Reactivities | Rat, Human, Pig |
| Antigen | Purified human skin Chymase |
| Description | Mouse Monoclonal |
| Gene | CMA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2377 - immunohistochemistry Formalin fixed paraffin embedded human tonsil stained with Mast Cell Chymase using ABC and AEC chromogen.
![Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1/250 dilution + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 35 kDa (why is the actual band size different from the predicted?)Exposure time : 8 minutes](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19775_Mast-Cell-Chymase-Primary-antibodies-ab2377-2.jpg)
Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1/250 dilution + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 35 kDa (why is the actual band size different from the predicted?)Exposure time : 8 minutes
![Overlay histogram showing A431 cells stained with ab2733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19776_Mast-Cell-Chymase-Primary-antibodies-ab2377-4.jpg)
Overlay histogram showing A431 cells stained with ab2733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute - Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute
Zheng J, Wei CC, Hase N, Shi K, Killingsworth CR, Litovsky SH, Powell PC, Kobayashi T, Ferrario CM, Rab A, Aban I, Collawn JF, Dell'Italia LJ. PLoS One. 2014 Apr 14;9(4):e94732.
Altered immunohistochemical expression of mast cell tryptase and chymase in the - Altered immunohistochemical expression of mast cell tryptase and chymase in the
Yadav A, Desai RS, Bhuta BA, Singh JS, Mehta R, Nehete AP. PLoS One. 2014 May 29;9(5):e98719.
Stress management affects outcomes in the pathophysiology of an endometriosis - Stress management affects outcomes in the pathophysiology of an endometriosis
Appleyard CB, Cruz ML, Hernandez S, Thompson KJ, Bayona M, Flores I. Reprod Sci. 2015 Apr;22(4):431-41.
Cellular immune environment in endometrial polyps. - Cellular immune environment in endometrial polyps.
El-Hamarneh T, Hey-Cunningham AJ, Berbic M, Al-Jefout M, Fraser IS, Black K. Fertil Steril. 2013 Nov;100(5):1364-72.
Mast cells in COPD airways: relationship to bronchodilator responsiveness and - Mast cells in COPD airways: relationship to bronchodilator responsiveness and
Soltani A, Ewe YP, Lim ZS, Sohal SS, Reid D, Weston S, Wood-Baker R, Walters EH. Eur Respir J. 2012 Jun;39(6):1361-7.
Chymase inhibition reduces infarction and matrix metalloproteinase-9 activation - Chymase inhibition reduces infarction and matrix metalloproteinase-9 activation
Oyamada S, Bianchi C, Takai S, Chu LM, Sellke FW. J Pharmacol Exp Ther. 2011 Oct;339(1):143-51.
Impact of acute myocardial ischemia reperfusion on the tissue and blood-borne - Impact of acute myocardial ischemia reperfusion on the tissue and blood-borne
Oyamada S, Bianchi C, Takai S, Robich MP, Clements RT, Chu L, Sellke FW. Basic Res Cardiol. 2010 Jul;105(4):513-22.
Alpha 2-macroglobulin capture allows detection of mast cell chymase in serum and - Alpha 2-macroglobulin capture allows detection of mast cell chymase in serum and
Raymond WW, Su S, Makarova A, Wilson TM, Carter MC, Metcalfe DD, Caughey GH. J Immunol. 2009 May 1;182(9):5770-7.
Human mast cells express leptin and leptin receptors. - Human mast cells express leptin and leptin receptors.
Taildeman J, Perez-Novo CA, Rottiers I, Ferdinande L, Waeytens A, De Colvenaer V, Bachert C, Demetter P, Waelput W, Braet K, Cuvelier CA. Histochem Cell Biol. 2009 Jun;131(6):703-11.
Activation of the intracellular renin-angiotensin system in cardiac fibroblasts - Activation of the intracellular renin-angiotensin system in cardiac fibroblasts
Singh VP, Baker KM, Kumar R. Am J Physiol Heart Circ Physiol. 2008 Apr;294(4):H1675-84. doi: