Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling MDH1 with ab181091 at 1/100 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-MDH1 antibody [EPR13596(B)] (ab181091) at 1/2000 dilutionLane 1 : Human spleen lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution