Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH2 with ab181857 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling MDH2 with ab181857 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-MDH2 antibody [EPR14883(B)] (ab181857) at 1/50000 dilutionLane 1 : K562 cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution