Anti-ME2 antibody [EP7217]

• Supplier: Abcam
• Catalog: ab139686
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP7217
• Applications: WB ICC/IF ICC/IF IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: ME2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab139686 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139686, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-ME2 antibody [EP7217] (ab139686) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : HL60 cell lysateLane 4 : 293T cell lysateLane 5 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue labeling ME2 with ab139686 at 1/50 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ME2 with ab139686 at 1/50 dilution.

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab139686 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139686 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab139686 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab139686 showing +ve staining.