Anti-MEK2 (phospho T394) antibody
| Name | Anti-MEK2 (phospho T394) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30622 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide derived from human MEK2 around the phosphorylation site of Threonine 394 |
| Description | Rabbit Polyclonal |
| Gene | MAP2K2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MEK2 (phospho T394) antibody (ab30622) at 1/500 dilutionLane 1 : extracts from ovary cancer cells.Lane 2 : extracts from ovary cancer cells, preincubated with synthesized non phosphopeptideLane 3 : extracts from ovary cancer cells, preincubated with synthesized phosphopeptide (negative control).
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 MEK2 (Phospho Thr394) Antibody (ab30622). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
ICC/IF image of ab30622 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.