Anti-MMP9 antibody

• Supplier: Abcam
• Catalog: ab7299
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F IP WB IHC-P
• Species Reactivities: Rat, Human
• Antigen: Recombinant fragment (catalytic domain) (Rat)
• Description: Rabbit Polyclonal
• Gene: MMP9
• Conjugate: Unconjugated

Product images

IHC image of MMP9 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab7299, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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