Anti-mTOR antibody [Y391]
| Name | Anti-mTOR antibody [Y391] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32028 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y391 |
| Applications | ChIP WB ICC/IF ICC/IF IP FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen |
| Blocking Peptide | mTOR peptide |
| Description | Rabbit Monoclonal |
| Gene | MTOR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab32028 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32028, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
Immunohistochemical analysis of mTOR expression in paraffin-embedded human prostate carcinoma, using 1/250 ab32028.
ICC/IF image of ab32028 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32028, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-mTOR antibody [Y391] (ab32028) at 1/2000 dilutionLane 1 : Hela cell lysateLane 2 : MCF-7](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25677_ab32028wb.jpg)
All lanes : Anti-mTOR antibody [Y391] (ab32028) at 1/2000 dilutionLane 1 : Hela cell lysateLane 2 : MCF-7
Product References
Activation of the PI3K/mTOR/AKT pathway and survival in solid tumors: systematic - Activation of the PI3K/mTOR/AKT pathway and survival in solid tumors: systematic
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Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and - Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and
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Overexpression of phosphorylated mammalian target of rapamycin predicts lymph - Overexpression of phosphorylated mammalian target of rapamycin predicts lymph
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The role of mTOR and phospho-p70S6K in pathogenesis and progression of gastric - The role of mTOR and phospho-p70S6K in pathogenesis and progression of gastric
Xiao L, Wang YC, Li WS, Du Y. J Exp Clin Cancer Res. 2009 Dec 13;28:152.
Systematic reviews for the Journal of Experimental & Clinical Cancer Research: - Systematic reviews for the Journal of Experimental & Clinical Cancer Research:
Schunemann HJ, Castelli M, Muti P. J Exp Clin Cancer Res. 2007 Jun;26(2):169-74.