Anti-Musashi 1 / Msi1 antibody [EP1302]
| Name | Anti-Musashi 1 / Msi1 antibody [EP1302] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52865 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1302 |
| Applications | IHC-P IHC-F WB IP ICC/IF FC |
| Species Reactivities | Chicken, Human, Quail |
| Antigen | Synthetic peptide corresponding to residues on the N terminal of human Musashi 1/ Msi1 |
| Blocking Peptide | Musashi 1 / Msi1 peptide |
| Description | Rabbit Monoclonal |
| Gene | MSI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution + SH-SY-5Y cell lysate at 10 µg/mlSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26129_ab52865%2520wb.bmp)
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution + SH-SY-5Y cell lysate at 10 µg/mlSecondarygoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical staining of paraffin embedded human brain using ab52865 (1/50-1/100 dilution).
Immunofluorescent staining of PC12 cells using ab52865 (1/250-1/500 dilution).
![Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26132_ab52865_id9265_reduced.jpg)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized. The cells were incubated with ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.See Abreview
![Musashi 1 / Msi1 was immunoprecipitated using 0.5mg SHSY5Y whole cell extract, 10µg of Rabbit monoclonal to Musashi 1 / Msi1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, SHSY5Y whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52865.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 39kDa: Musashi 1 / Msi1; non specific - 29kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26134_Musashi-1-Msi1-Primary-antibodies-ab52865-17.jpg)
Musashi 1 / Msi1 was immunoprecipitated using 0.5mg SHSY5Y whole cell extract, 10µg of Rabbit monoclonal to Musashi 1 / Msi1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, SHSY5Y whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52865.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 39kDa: Musashi 1 / Msi1; non specific - 29kDa: We are unsure as to the identity of this extra band.
Product References
Multiple tumor suppressor microRNAs regulate telomerase and TCF7, an important - Multiple tumor suppressor microRNAs regulate telomerase and TCF7, an important
Hrdlickova R, Nehyba J, Bargmann W, Bose HR Jr. PLoS One. 2014 Feb 14;9(2):e86990.
Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early - Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early
Femia AP, Dolara P, Salvadori M, Caderni G. BMC Cancer. 2013 Feb 1;13:48.
Expression and significance of Musashi-1 in gastric cancer and precancerous - Expression and significance of Musashi-1 in gastric cancer and precancerous
Kuang RG, Kuang Y, Luo QF, Zhou CJ, Ji R, Wang JW. World J Gastroenterol. 2013 Oct 21;19(39):6637-44.
The oncogenic RNA-binding protein Musashi1 is regulated by HuR via mRNA - The oncogenic RNA-binding protein Musashi1 is regulated by HuR via mRNA
Vo DT, Abdelmohsen K, Martindale JL, Qiao M, Tominaga K, Burton TL, Gelfond JA, Brenner AJ, Patel V, Trageser D, Scheffler B, Gorospe M, Penalva LO. Mol Cancer Res. 2012 Jan;10(1):143-55.
A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by - A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by
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Epithelial-connective tissue interactions induced by thyroid hormone receptor are - Epithelial-connective tissue interactions induced by thyroid hormone receptor are
Hasebe T, Buchholz DR, Shi YB, Ishizuya-Oka A. Stem Cells. 2011 Jan;29(1):154-61.
Lymphoid aggregates may contribute to the migration and epithelial commitment of - Lymphoid aggregates may contribute to the migration and epithelial commitment of
Valcz G, Krenacs T, Sipos F, Patai AV, Wichmann B, Leiszter K, Toth K, Balogh Z, Csizmadia A, Hagymasi K, Masszi T, Molnar B, Tulassay Z. J Clin Pathol. 2011 Sep;64(9):771-5.
Inhibition of glioblastoma growth by the thiadiazolidinone compound TDZD-8. - Inhibition of glioblastoma growth by the thiadiazolidinone compound TDZD-8.
Aguilar-Morante D, Morales-Garcia JA, Sanz-SanCristobal M, Garcia-Cabezas MA, Santos A, Perez-Castillo A. PLoS One. 2010 Nov 8;5(11):e13879.