Anti-Myosin VIIa antibody

• Supplier: Abcam
• Catalog: ab3481
• Prices: $400.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF IP WB ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Guinea Pig, Hamster, Bovine, Dog, Human, Pig
• Antigen: Synthetic peptide corresponding to Mouse Myosin VIIa aa 16-31
• Description: Rabbit Polyclonal
• Gene: MYO7A
• Conjugate: Unconjugated

Product images

Western blot detection of Myosin VIIa in mouse testes tissue extract using ab3481.

ab3481 staining primary cell culture of Pig retinal pigment epithelium by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 148 conjugated goat anti-rabbit antibody, diluted 1/750, was used as the secondary.See Abreview

ab3481 at a 1/500 dilution staining Myosin VIIa in Mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C in 1% goat serum, 0.1% Triton X-100, 1X PBS. Fixed in formalin. Permeabilized using 0.5% Triton X-100. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at 1/500 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.See Abreview

(A) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin.  (B) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with ab3481 as the primary antibody (dilution 1/900) and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

(G) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (H) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with rabbit IgG as an isotype control and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

Product References

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