β-Catenin (D10A8) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8480
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D10A8
• Applications: WB IP IHC-P IHC-F ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Zebrafish, Bovine, Pig, Guinea Pig, Horse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.
• Description: Rabbit Monoclonal
• Gene: CTNNB1

Product images

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP ® Rabbit mAb.

Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of NCI-H28 (blue) or HeLa (green) cells using β-Catenin (D10A8) XP ® Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT116 cells and either 20 μl of β-Catenin (D10A8) XP ® Rabbit mAb or 5 μl of Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. The figure shows binding across AXIN2, a known target gene of β-Catenin (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT 116 cells and either 20 μl of β-Catenin (D10A8) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human Axin2 Intron 1 Primers #8973, SimpleChIP ® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.