β-Catenin Antibody (Amino-terminal Antigen)
| Name | β-Catenin Antibody (Amino-terminal Antigen) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9581 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin |
| Description | Rabbit Polyclonal |
| Gene | CTNNB1 |
| Conjugate | Amino-terminal Antigen |
| Supplier Page | Shop |
Product images
Western blot analysis of total cell extracts from 293 and NIH/3T3 cells, using β-Catenin Antibody (Amino-terminal Antigen).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT116 cells and either 10 μl of β-Catenin Antibody (Amino-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP ® Human Axin2 Intron 1 Primers #8973, SimpleChIP ® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.