Anti-NQO1 antibody
| Name | Anti-NQO1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34173 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P IHC-F WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Guinea Pig, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human NQO1 |
| Description | Rabbit Polyclonal |
| Gene | NQO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µgSecondaryIRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlas . ab34173 staining NQO1 in human breast cancer. Paraffin embedded human breast cancer tissue was incubated with ab34173 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab34173 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the cature antibody and ab34173 (1/1000) for the detection. Please refer to abreview for further experimental details.See Abreview
Anti-NQO1 antibody (ab34173) at 1 µg/ml + Active human NQO1 full length protein (ab59663) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
ICC/IF image of ab34173 stained HeLa cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34173, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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