Anti-NQO1 antibody
| Name | Anti-NQO1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2346 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F ELISA IHC-F |
| Species Reactivities | Mouse, Rat, Dog, Human, Pig |
| Antigen | Synthetic peptide: SIPTDNQIKARK, corresponding to C terminal amino acids 267-274 of Human NQO1 |
| Description | Goat Polyclonal |
| Gene | NQO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346.
Anti-NQO1 antibody (ab2346) at 0.03 µg/ml + Human Kidney lysate at 35 µgObserved band size : 30 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
NRF2 immunolocalization in human breast cancer patients as a prognostic factor. - NRF2 immunolocalization in human breast cancer patients as a prognostic factor.
Onodera Y, Motohashi H, Takagi K, Miki Y, Shibahara Y, Watanabe M, Ishida T, Hirakawa H, Sasano H, Yamamoto M, Suzuki T. Endocr Relat Cancer. 2014 Feb 27;21(2):241-52.
Nrf2 enhances cholangiocyte expansion in Pten-deficient livers. - Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
Taguchi K, Hirano I, Itoh T, Tanaka M, Miyajima A, Suzuki A, Motohashi H, Yamamoto M. Mol Cell Biol. 2014 Mar;34(5):900-13.
Overexpression of Nrf2 protects against microcystin-induced hepatotoxicity in - Overexpression of Nrf2 protects against microcystin-induced hepatotoxicity in
Lu YF, Liu J, Wu KC, Qu Q, Fan F, Klaassen CD. PLoS One. 2014 Mar 25;9(3):e93013.
NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked - NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked
Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, Catanzaro JM, Friedman SL, Zong WX, Fiel MI, Li M, Yue Z. Nat Commun. 2014 May 22;5:3920.
Identification and quantification of the basal and inducible Nrf2-dependent - Identification and quantification of the basal and inducible Nrf2-dependent
Walsh J, Jenkins RE, Wong M, Olayanju A, Powell H, Copple I, O'Neill PM, Goldring CE, Kitteringham NR, Park BK. J Proteomics. 2014 Aug 28;108:171-87.
Is nuclear factor erythroid 2-related factor 2 responsible for sex differences in - Is nuclear factor erythroid 2-related factor 2 responsible for sex differences in
Rohrer PR, Rudraiah S, Goedken MJ, Manautou JE. Drug Metab Dispos. 2014 Oct;42(10):1663-74.
Dishevelled proteins are associated with olfactory sensory neuron presynaptic - Dishevelled proteins are associated with olfactory sensory neuron presynaptic
Rodriguez-Gil DJ, Hu W, Greer CA. PLoS One. 2013;8(2):e56561.
Oleanolic acid alters bile acid metabolism and produces cholestatic liver injury - Oleanolic acid alters bile acid metabolism and produces cholestatic liver injury
Liu J, Lu YF, Zhang Y, Wu KC, Fan F, Klaassen CD. Toxicol Appl Pharmacol. 2013 Nov 1;272(3):816-24. doi:
Coenzyme Q(1) as a probe for mitochondrial complex I activity in the intact - Coenzyme Q(1) as a probe for mitochondrial complex I activity in the intact
Bongard RD, Myers CR, Lindemer BJ, Baumgardt S, Gonzalez FJ, Merker MP. Am J Physiol Lung Cell Mol Physiol. 2012 May 1;302(9):L949-58. doi:
Inducible disruption of autophagy in the lung causes airway hyper-responsiveness. - Inducible disruption of autophagy in the lung causes airway hyper-responsiveness.
Inoue D, Kubo H, Taguchi K, Suzuki T, Komatsu M, Motohashi H, Yamamoto M. Biochem Biophys Res Commun. 2011 Feb 4;405(1):13-8. doi: