Anti-NSF antibody [NSF-1]

Name	Anti-NSF antibody [NSF-1]
Supplier	Abcam
Catalog	ab16681
Prices	$395.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone	NSF-1
Applications	ICC/IF ICC/IF WB FC IP IHC-P
Species Reactivities	Mouse, Rat, Chicken, Bovine, Human
Antigen	Recombinant full length protein (Human)
Description	Mouse Monoclonal
Gene	NSF
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-NSF antibody [NSF-1] (ab16681) at 0.5 µg/mlLane 1 : 3T3Lane 2 : PC12Lane 3 : MDBKLane 4 : Normal human fibroblasts

ICC/IF image of ab16681 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16681, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing SH-SY5Y cells stained with ab16681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16681, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

Proteomic changes in rat hippocampus and adrenals following short-term sleep - Proteomic changes in rat hippocampus and adrenals following short-term sleep

Poirrier JE, Guillonneau F, Renaut J, Sergeant K, Luxen A, Maquet P, Leprince P. Proteome Sci. 2008 May 22;6:14.

SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated - SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated

Bostrom P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Boren J, Olofsson SO. Nat Cell Biol. 2007 Nov;9(11):1286-93. Epub 2007 Oct 7.