Anti-Nucleobindin 2 antibody [EPR13159]

• Supplier: Abcam
• Catalog: ab181159
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13159
• Applications: WB IHC-P ICC/IF ICC/IF FC IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nucleobindin 2 aa 1-100
• Description: Rabbit Monoclonal
• Gene: NUCB2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Nucleobindin 2 antibody [EPR13159] (ab181159) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HepG2 cell lysateLane 3 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Nucleobindin 2 with ab181159 at 1/500 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling Nucleobindin 2 with ab181159 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Nucleobindin 2 with ab181159 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Western blot analysis of Nucleobindin 2 in Human stomach lysate immunoprecipitated with ab181159 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.