Anti-OPA1 antibody [1E81D9]
| Name | Anti-OPA1 antibody [1E81D9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab119685 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1E81D9 |
| Applications | WB FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Human, full-length, corresponding to short form of isoform 1 |
| Description | Mouse Monoclonal |
| Gene | OPA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3858_ab119685-2-ab119685FC.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/mlLane 1 : whole cell lysates from HeLa cells(human)Lane 2 : whole cell lysates from H4IIE cells(rat)Lane 3 : whole cell lysates from MEF cells(mouse)Lysates/proteins at 30 µg per lane.SecondaryHRP goat anti-mouse at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3859_OPA1-Primary-antibodies-ab119685-2.jpg)
All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/mlLane 1 : whole cell lysates from HeLa cells(human)Lane 2 : whole cell lysates from H4IIE cells(rat)Lane 3 : whole cell lysates from MEF cells(mouse)Lysates/proteins at 30 µg per lane.SecondaryHRP goat anti-mouse at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.