Anti-PBR antibody [EPR5384]
| Name | Anti-PBR antibody [EPR5384] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109497 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5384 |
| Applications | WB IP IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide corresponding to residues at the C-terminus of Human PBR |
| Blocking Peptide | PBR peptide |
| Description | Rabbit Monoclonal |
| Gene | TSPO |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PBR antibody [EPR5384] (ab109497) at 1/10000 dilutionLane 1 : U-87 MG cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLane 4 : RAW264.7 cell lysateLane 5 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7982_PBR-Primary-antibodies-ab109497-1.jpg)
All lanes : Anti-PBR antibody [EPR5384] (ab109497) at 1/10000 dilutionLane 1 : U-87 MG cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLane 4 : RAW264.7 cell lysateLane 5 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.
ab109497 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
ab109497 at 1/50 dilution staining PBR in A431 cells
Overlay histogram showing HepG2 cells stained with ab109497 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109497, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Acute neuroinflammation in a clinically relevant focal cortical ischemic stroke - Acute neuroinflammation in a clinically relevant focal cortical ischemic stroke
Toth M, Little P, Arnberg F, Haggkvist J, Mulder J, Halldin C, Gulyas B, Holmin S. Brain Struct Funct. 2015 Jan 20.
Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia - Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia
Karlstetter M, Nothdurfter C, Aslanidis A, Moeller K, Horn F, Scholz R, Neumann H, Weber BH, Rupprecht R, Langmann T. J Neuroinflammation. 2014 Jan 8;11:3.
Macroglia-microglia interactions via TSPO signaling regulates microglial - Macroglia-microglia interactions via TSPO signaling regulates microglial
Wang M, Wang X, Zhao L, Ma W, Rodriguez IR, Fariss RN, Wong WT. J Neurosci. 2014 Mar 5;34(10):3793-806.
Regulation of S100B in white adipose tissue by obesity in mice. - Regulation of S100B in white adipose tissue by obesity in mice.
Buckman LB, Anderson-Baucum EK, Hasty AH, Ellacott KLj. Adipocyte. 2014 Jul 1;3(3):215-20.
Peripheral benzodiazepine receptor/translocator protein global knock-out mice are - Peripheral benzodiazepine receptor/translocator protein global knock-out mice are
Tu LN, Morohaku K, Manna PR, Pelton SH, Butler WR, Stocco DM, Selvaraj V. J Biol Chem. 2014 Oct 3;289(40):27444-54.
IF1 limits the apoptotic-signalling cascade by preventing mitochondrial - IF1 limits the apoptotic-signalling cascade by preventing mitochondrial
Faccenda D, Tan CH, Seraphim A, Duchen MR, Campanella M. Cell Death Differ. 2013 May;20(5):686-97.
Developmental expression of translocator protein/peripheral benzodiazepine - Developmental expression of translocator protein/peripheral benzodiazepine
Morohaku K, Phuong NS, Selvaraj V. PLoS One. 2013 Sep 5;8(9):e74509.
Translocator protein 18 kDa (TSPO) is regulated in white and brown adipose tissue - Translocator protein 18 kDa (TSPO) is regulated in white and brown adipose tissue
Thompson MM, Manning HC, Ellacott KL. PLoS One. 2013 Nov 18;8(11):e79980.