Anti-PERK antibody
| Name | Anti-PERK antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65142 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF WB IP |
| Species Reactivities | Human |
| Antigen | A synthesized peptide derived from an internal region of human PERK |
| Description | Rabbit Polyclonal |
| Gene | EIF2AK3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of HepG2 cells using ab65142 at a 1/500-1/1000 dilution. Left image untreated. Right image treated with immunising peptide.
All lanes : Anti-PERK antibody (ab65142) at 1/500 dilutionLane 1 : Extracts from Hela cellsLane 2 : Extracts from HepG2 cellsLane 3 : Extracts from HepG2 cells, plus immunising peptide
![PERK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PERK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65142.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 130kDa: PERK.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/9979_PERK-Primary-antibodies-ab65142-4.jpg)
PERK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PERK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65142.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 130kDa: PERK.
Product References
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Protein kinase CK2 protects multiple myeloma cells from ER stress-induced - Protein kinase CK2 protects multiple myeloma cells from ER stress-induced
Manni S, Brancalion A, Tubi LQ, Colpo A, Pavan L, Cabrelle A, Ave E, Zaffino F, Di Maira G, Ruzzene M, Adami F, Zambello R, Pitari MR, Tassone P, Pinna LA, Gurrieri C, Semenzato G, Piazza F. Clin Cancer Res. 2012 Apr 1;18(7):1888-900.