IHC-P image of PERK staining on human cortical sections using ab77654 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab77654 was diluted 1:500 and incubated with the sections for 18 hours at 4°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500) See Abreview
Formalin fixed and paraffin embedded human hepatocarcinoma tissue labelled with ab77654 (at a 1/50 dilution), which was peroxidase conjugated to the secondary antibody, followed by DAB staining.