Anti-Phospholipase A2 antibody

• Supplier: Abcam
• Catalog: ab23705
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB IP
• Species Reactivities: Rat, Human
• Antigen: Human recombinant sPLA2 (Type IIa)
• Description: Rabbit Polyclonal
• Gene: PLA2G2A
• Conjugate: Unconjugated

Product images

ab23705 at 1/1000 western blot detection of 0.25 ug of human secretory phospolipase A2.

IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host - Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host

Pernet E, Guillemot L, Burgel PR, Martin C, Lambeau G, Sermet-Gaudelus I, Sands D, Leduc D, Morand PC, Jeammet L, Chignard M, Wu Y, Touqui L. Nat Commun. 2014 Oct 7;5:5105.

Crucial role of group IIA phospholipase A2 in pancreatitis-associated adrenal - Crucial role of group IIA phospholipase A2 in pancreatitis-associated adrenal

Xu S, Chen C, Wang WX, Huang SR, Yu J, Chen XY. Pathol Res Pract. 2010 Feb 15;206(2):73-82.