Anti-PIM1 antibody [EP2645Y]
| Name | Anti-PIM1 antibody [EP2645Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75776 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2645Y |
| Applications | ICC/IF ICC/IF WB IHC-P ICC/IF |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | A synthetic peptide corresponding to residues in human PIM1 |
| Description | Rabbit Monoclonal |
| Gene | PIM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-PIM1 antibody [EP2645Y] (ab75776) at 1/20000 dilution + Jurkat at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12002_ab75776-223997-ab75776.jpg)
Anti-PIM1 antibody [EP2645Y] (ab75776) at 1/20000 dilution + Jurkat at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
ab75776 at 1/100-1/250 dilution staining PIM1 in human prostatic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
ICC/IF image of ab75776 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75776, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab75776 showing positive staining in Thyroid gland carcinoma tissue.
ab75776 showing positive staining in Stomach adenocarcinoma tissue.
ab75776 showing positive staining in Colonic adenocarcinoma tissue.
ab75776 showing positive staining in Breast carcinoma tissue.
ab75776 showing positive staining in Normal tonsil tissue.
Product References
Pim1 kinase is upregulated in glioblastoma multiforme and mediates tumor cell - Pim1 kinase is upregulated in glioblastoma multiforme and mediates tumor cell
Herzog S, Fink MA, Weitmann K, Friedel C, Hadlich S, Langner S, Kindermann K, Holm T, Bohm A, Eskilsson E, Miletic H, Hildner M, Fritsch M, Vogelgesang S, Havemann C, Ritter CA, Meyer zu Schwabedissen HE, Rauch B, Hoffmann W, Kroemer HK, Schroeder H, Bien-Moller S. Neuro Oncol. 2015 Feb;17(2):223-42.
Tricyclic Benzo[cd]azulenes selectively inhibit activities of Pim kinases and - Tricyclic Benzo[cd]azulenes selectively inhibit activities of Pim kinases and
Kiriazis A, Vahakoski RL, Santio NM, Arnaudova R, Eerola SK, Rainio EM, Aumuller IB, Yli-Kauhaluoma J, Koskinen PJ. PLoS One. 2013;8(2):e55409.
Conditional transgenic expression of PIM1 kinase in prostate induces - Conditional transgenic expression of PIM1 kinase in prostate induces
Narlik-Grassow M, Blanco-Aparicio C, Cecilia Y, Perez M, Munoz-Galvan S, Canamero M, Renner O, Carnero A. PLoS One. 2013;8(4):e60277.
Functional role and therapeutic potential of the pim-1 kinase in colon carcinoma. - Functional role and therapeutic potential of the pim-1 kinase in colon carcinoma.
Weirauch U, Beckmann N, Thomas M, Grunweller A, Huber K, Bracher F, Hartmann RK, Aigner A. Neoplasia. 2013 Jul;15(7):783-94.
Expression of pim-1 in tumors, tumor stroma and tumor-adjacent mucosa - Expression of pim-1 in tumors, tumor stroma and tumor-adjacent mucosa
Peng YH, Li JJ, Xie FW, Chen JF, Yu YH, Ouyang XN, Liang HJ. PLoS One. 2013 Oct 7;8(10):e76693.
Expression of proviral integration site for Moloney murine leukemia virus 1 - Expression of proviral integration site for Moloney murine leukemia virus 1
Kim HK, Kim CW, Vo MT, Lee HH, Lee JY, Yoon NA, Lee CY, Moon CH, Min YJ, Park JW, Cho WJ. J Biol Chem. 2012 Aug 17;287(34):28770-8.
Clinical and therapeutic relevance of PIM1 kinase in gastric cancer. - Clinical and therapeutic relevance of PIM1 kinase in gastric cancer.
Yan B, Yau EX, Samanta S, Ong CW, Yong KJ, Ng LK, Bhattacharya B, Lim KH, Soong R, Yeoh KG, Deng N, Tan P, Lam Y, Salto-Tellez M. Gastric Cancer. 2012 Apr;15(2):188-97.
Phosphorylation of the androgen receptor by PIM1 in hormone refractory prostate - Phosphorylation of the androgen receptor by PIM1 in hormone refractory prostate
Ha S, Iqbal NJ, Mita P, Ruoff R, Gerald WL, Lepor H, Taneja SS, Lee P, Melamed J, Garabedian MJ, Logan SK. Oncogene. 2013 Aug 22;32(34):3992-4000.