Anti-PKC alpha + beta + gamma antibody [EPR16898]

• Supplier: Abcam
• Catalog: ab179522
• Prices: $99.00, $357.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16898
• Applications: WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human PKC alpha + beta + gamma aa 300-500
• Description: Rabbit Monoclonal
• Gene: PRKCA
• Conjugate: Unconjugated

Product images

All lanes : Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µgLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µgLane 3 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/1000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µgLane 2 : A431 (Human epidermoid carcinoma) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/5000 dilutionLane 1 : L6 (Rat skeletal muscle cell line) whole cell lysateLane 2 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysateLane 3 : L929 (Mouse connective tissue fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/1000 dilution + Rat brain whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PKC alpha + beta + gamma with ab179522 at 1/200 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).The negative controls are as follows:--ve control 1: - ab179522 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed NIH/3T3 (Mouse embyro fibroblast cells) cells labeling PKC alpha + beta + gamma with ab179522 at 1/360 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

PKC alpha + beta + gamma was immunoprecipitated from 1mg of Rat brain extract with ab179522 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab179522 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody was used as secondary antibody at 1/1000 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

PKC alpha + beta + gamma was immunoprecipitated from 1mg of Rat brain extract with ab179522 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab108961 (Rabbit monoclonal [EPR2750] to PKC gamma) at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody was used as secondary antibody at 1/1000 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST.

PKC alpha + beta + gamma was immunoprecipitated from 1mg of Rat brain extract with ab179522 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab32376 (Rabbit monoclonal [Y124] to PKC alpha) at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody was used as secondary antibody at 1/1000 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST.