Anti-POLD3 antibody [EP9480] (ab182564) at 1/5000 dilution + Jurkat lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-POLD3 antibody [EP9480] (ab182564) at 1/5000 dilutionLane 1 : Hela lysateLane 2 : 293T lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling POLD3 with ab182564 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (left panel). Right panel counter stained with Dapi (blue).