Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3D with ab86786 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-POLR3D antibody (ab86786) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of POLR3D by Western Blot of Immunprecipitate. ab86786, at 1 µg/ml, staining POLR3D in HeLa whole cell lysate immunoprecipitated using ab86786 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.