Anti-PPAR gamma antibody [3A4A9,1E6A1]
| Name | Anti-PPAR gamma antibody [3A4A9,1E6A1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab70405 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3A4A9,1E6A1 |
| Applications | WB ELISA FC |
| Species Reactivities | Sheep, Human, Mouse, Rat, Rabbit, Horse, Chicken, Bovine, Cat, Dog, Pig, Chimpanzee, Monkey, Monkey, Hamster |
| Antigen | Recombinant fragment: KLIYDRCDLN CRIHKKSRNK CQYCRFQKCL AVGMSHNAIR FGRMPQAEKE KLLAEISSDI DQLNPESADL RALAKHLYDS YIKSFPLTKA KARAILTGKT T, corresponding to amino acids 170-270 of Human PPAR gamma with His tag expressed in E |
| Description | Mouse Monoclonal |
| Gene | PPARG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PPAR gamma antibody [3A4A9,1E6A1] (ab70405) at 1/500 dilutionLane 1 : Molecular weight markerLane 2 : Truncated PPAR gamma-His recombinant proteinLane 3 : Full length PPAR gamma transfected CHO-K1 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14799_ab70405-1.jpg)
All lanes : Anti-PPAR gamma antibody [3A4A9,1E6A1] (ab70405) at 1/500 dilutionLane 1 : Molecular weight markerLane 2 : Truncated PPAR gamma-His recombinant proteinLane 3 : Full length PPAR gamma transfected CHO-K1 cell lysate
![Overlay histogram showing HepG2 cells stained with ab70405 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70405, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14800_PPAR-gamma-Primary-antibodies-ab70405-1.jpg)
Overlay histogram showing HepG2 cells stained with ab70405 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70405, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.