To detect Murine SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine SDF-1-alpha (XP-5270Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine SDF-1-alpha.
To detect Murine SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.