All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilutionLane 1 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with NO added peptideLane 2 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with non-phosphopeptide corresponding to the immunogenLane 3 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with generic phosphothreonine containing peptide Lane 4 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with phosphopeptide immunogenSecondaryF(ab')2 ant-rabbit IgG HRP conjugatedeveloped using the ECL technique
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilutionLane 1 : HeLa cell extract with NO nocodazoleLane 2 : HeLa cell extract with 200ng/ml nocodazole for 16 hoursLane 3 : RAW 264.7 cell extract with NO nocodazoleLane 4 : RAW 264.7 cell extract with 200ng/ml nocodazole for 16 hoursLane 5 : HeLa cell extract with an antibody recognizing total human IPP2 protein Lane 6 : HeLa cell extract with an antibody recognizing total human IPP2 protein SecondaryF(ab')2 ant-rabbit IgG HRP conjugatedeveloped using the ECL techniquePerformed under reducing conditions.
