Anti-PTOP antibody
| Name | Anti-PTOP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57595 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-545 of Human PTOP |
| Description | Mouse Monoclonal |
| Gene | ACD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
PTOP antibody (ab57595) used in immunofluorescence at 10ug/ml on HeLa cells.
PTOP antibody (ab57595) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human heart tissue.
![Overlay histogram showing HeLa cells stained with ab57595 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57595, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18412_PTOP-Primary-antibodies-ab57595-1.jpg)
Overlay histogram showing HeLa cells stained with ab57595 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57595, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
PTOP and TRF1 help enhance the radio resistance in breast cancer cell. - PTOP and TRF1 help enhance the radio resistance in breast cancer cell.
Li Z, Yang X, Xia N, Yang L, Yu H, Zhou F, X C, Zhou Y. Cancer Cell Int. 2014 Jan 25;14(1):7.
Sirtuin 1 is upregulated in a subset of hepatocellular carcinomas where it is - Sirtuin 1 is upregulated in a subset of hepatocellular carcinomas where it is
Chen J, Zhang B, Wong N, Lo AW, To KF, Chan AW, Ng MH, Ho CY, Cheng SH, Lai PB, Yu J, Ng HK, Ling MT, Huang AL, Cai XF, Ko BC. Cancer Res. 2011 Jun 15;71(12):4138-49.