Anti-Pyruvate Dehydrogenase E1 beta subunit antibody
| Name | Anti-Pyruvate Dehydrogenase E1 beta subunit antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55574 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 250-360 of Human Pyruvate Dehydrogenase E1 beta subunit |
| Description | Mouse Monoclonal |
| Gene | PDHB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Pyruvate Dehydrogenase E1 beta subunit antibody (ab55574) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.
![Overlay histogram showing HepG2 cells stained with ab55574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55574, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19038_Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab55574-2.jpg)
Overlay histogram showing HepG2 cells stained with ab55574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55574, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as - Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as
Xie LQ, Zhao C, Cai SJ, Xu Y, Huang LY, Bian JS, Shen CP, Lu HJ, Yang PY. J Proteome Res. 2010 Sep 3;9(9):4701-9.
Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant. - Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant.
Maurer CM, Schonthaler HB, Mueller KP, Neuhauss SC. J Neurosci. 2010 Sep 8;30(36):11962-72.