Anti-Rho GTPase-activating protein 24 antibody
| Name | Anti-Rho GTPase-activating protein 24 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab84046 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Rho GTPase-activating protein 24 |
| Description | Rabbit Polyclonal |
| Gene | ARHGAP24 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Rho GTPase-activating protein 24 antibody (ab84046) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab84046 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84046, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.