Anti-RhoA + C antibody [1B34A10]

• Supplier: Abcam
• Catalog: ab175359
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 1B34A10
• Applications: IP ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human
• Antigen: Recombinant full length protein corresponding to Human RhoA + C aa 1-193
• Description: Mouse Monoclonal
• Gene: RHOA
• Conjugate: Unconjugated

Product images

All lanes : Anti-RhoA + C antibody [1B34A10] (ab175359) at 1/1000 dilutionLane 1 : HeLa whole cell lysate at 25 µgLane 2 : A431 whole cell lysate at 25 µgLane 3 : HEK293T whole cell lysate at 25 µgLane 4 : U2 OS whole cell lysate at 25 µgLane 5 : NIH 3T3 whole cell lysate at 25 µgLane 6 : K562 whole cell lysate at 25 µgLane 7 : RhoA purified protein at 0.5 µgLane 8 : RhoB purified protein at 0.5 µgLane 9 : RhoC purified protein with proprietary tag at 0.5 µgSecondarygoat anti-mouse IgG-HRP at 1/15000 dilutiondeveloped using the ECL technique

Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling RhoA + C with ab175359 at 1/100 dilution followed with DyLight 488 goat anti-mouse IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

Immunoprecipitation analysis of RhoA + C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500μg of whole cell lysate with 2μg ab175359 (lane 2).HeLa cell lysate was run as a control (lane 1).For WB detection, ab175359 was used at 1/1000 dilution.