Anti-RhoA antibody
| Name | Anti-RhoA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68826 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F FPIA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Dog, Human, Chicken, Bovine, Xenopus, Zebrafish, Orangutan |
| Antigen | Synthetic peptide corresponding to RhoA |
| Description | Rabbit Polyclonal |
| Gene | RHOA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab68826 stained HeLa cells (ab150035). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68826 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-RhoA antibody (ab68826) at 1/2000 dilutionLane 2 : Anti-RhoA antibody (ab68826) at 1/2000 dilution (pre-absorbed with immunizing peptide)Lane 1 : Rat spinal cord lysateLane 2 : Rat spinal cord lysate
ab68826 (1/500) staining RhoA (red) and ab72019 staining Sortilin (green) in rat trigeminal tissue. DAPI counterstaining appears in blue.
Product References
Simvastatin modulates cellular components in influenza A virus-infected cells. - Simvastatin modulates cellular components in influenza A virus-infected cells.
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Human bone-lineage cell responses to anisotropic Ti6Al4V surfaces are dependent - Human bone-lineage cell responses to anisotropic Ti6Al4V surfaces are dependent
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Rho kinase activation mediates adrenergic and cholinergic smooth muscle - Rho kinase activation mediates adrenergic and cholinergic smooth muscle
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Endothelial cell-specific FGD5 involvement in vascular pruning defines neovessel - Endothelial cell-specific FGD5 involvement in vascular pruning defines neovessel
Cheng C, Haasdijk R, Tempel D, van de Kamp EH, Herpers R, Bos F, Den Dekker WK, Blonden LA, de Jong R, Burgisser PE, Chrifi I, Biessen EA, Dimmeler S, Schulte-Merker S, Duckers HJ. Circulation. 2012 Jun 26;125(25):3142-58.
Rab13-dependent trafficking of RhoA is required for directional migration and - Rab13-dependent trafficking of RhoA is required for directional migration and
Wu C, Agrawal S, Vasanji A, Drazba J, Sarkaria S, Xie J, Welch CM, Liu M, Anand-Apte B, Horowitz A. J Biol Chem. 2011 Jul 1;286(26):23511-20.
MPF governs the assembly and contraction of actomyosin rings by activating RhoA - MPF governs the assembly and contraction of actomyosin rings by activating RhoA
Wu YG, Zhou P, Lan GC, Gao D, Li Q, Wei DL, Wang HL, Tan JH. PLoS One. 2010 Sep 13;5(9):e12706.