
Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue labeling RNF7 withab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
![All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24058_ab181986-215434-ab181986wb2.jpg)
All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24059_ab181986-215433-ab181986wb1.jpg)
Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution