![All lanes : Anti-RON antibody [1B5A9] (ab76360) at 1/2000 dilutionLane 1 : Cell lysates prepared from HCC827 cellsLane 2 : Cell lysates prepared from HT-29 cellsLane 3 : Cell lysates prepared from HCT-116 cellsLane 4 : Cell lysates prepared from BxPC-3 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24307_RON-Primary-antibodies-ab76360-1.jpg)
All lanes : Anti-RON antibody [1B5A9] (ab76360) at 1/2000 dilutionLane 1 : Cell lysates prepared from HCC827 cellsLane 2 : Cell lysates prepared from HT-29 cellsLane 3 : Cell lysates prepared from HCT-116 cellsLane 4 : Cell lysates prepared from BxPC-3 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG1
![Overlay histogram showing THP1 cells stained with ab76360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76360, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24308_RON-Primary-antibodies-ab76360-2.jpg)
Overlay histogram showing THP1 cells stained with ab76360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76360, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.